An experiment to look at the effect of 1 factor on an enzyme

Aim To design and carry out an experiment to typeface at one factor which affects the enzyme catalase. Catalase is involved in the division of total heat Peroxide into water and atomic bite 8. 2H202 _ 2H20 + 02 It can be found in most cells, hitherto it is mostly concentrated in the liver, kidney and genus Tuber cells of plants (e.g. tater). Hypothesis I predict that as the temperature increases the atomic number 8 produced sink increase until a point (optimum temperature), then bequeath light until no oxygen is produced ( due to denaturation ) Justification All enzymes brace optimum conditions, at which they work best. therefrom in my experiment there result be an optimum temperature i.e. the temperature where oxygen is presumptuousness off most rapidly. I stated that at a temperature no oxygen would be produced. This is because I cogitate the enzyme entrust denature due to the high temperature ( ie the tertiary organize leave alone change ). Due to the denaturation of the enzyme, no oxygen impart be produced. I also feel it is important to swan note that the H2O2 is an unstable compound and at high temperatures (100aC) it will disintegrate by itself.

Apparatus For my experiment I will need the chase frame-up: h Conical flask with side on delivery outlet h stringent fitting bung for the conic flask h economy tube h Gas-syringe h Water bathroom h Retort stand h Boss and clamp I will set the apparatus up in the sideline way ¡V Control of variables FactorControl TemperatureI have elect to leave this factor using an electronic water bath in my experiment. ConcentrationI will use th! e substrate from the same bottleful without dilution. I will take my cuttings of potato from the same region. Surface AreaI will use the same cork borer and cut every(prenominal) of the potato to the... If you want to get a full essay, ordination it on our website: BestEssayCheap.com

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